The research group on Clinical Microbiology and Infectious diseases was created in 2004 after a cooperation research project together with the gastroenterologists of the Hospital Josep Trueta of Girona. The main goal of that collaboration was to study the bacterial composition of the colon from Crohn's Disease patients. We have also worked together with the laboratory of Microbiology of that Hospital to investigate the presence of Mycobacterium in the biopsies from those patients. Later, we started to cooperate with the Intensive Care Unit on two programs: one to detect Legionella pneumophila in patients admitted in this unit with severe community acquired pneumonia and the other to study the microbial ecology of the sepsis. After this time working with different aspects of bacterial infections, we are currently focusing our research interest in the Microbial Ecology of the Infection. Our hypothesis is that infections are not monospecific, but they could harbor novel bacteria.
Research lines:
Research subjects:
1. Microbial Ecology of the bacterial Infection
2. Microbiology of the Inflammatory Bowel Disease
Publications:
1. Martinez-Medina, M.; X. Aldeguer, F. Gonzàlez-Huix, L.J. Garcia-Gil. 2006. Abnormal microbiota composition in the ileo-colonic mucosa of Crohn’s disease patients as revealed by PCR-DGGE. Inflammatory Bowel Diseases 12:1136-1145
1.- Rodriguez-Lázaro, D., et al., Mycobacterium avium subsp. paratuberculosis not found in fresh ileocolonic mucosal biopsy specimens from patients with Crohn’s Disease using real-time PCR. Enviado a Microbial Pathogenesis.
Projects:
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Project:
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Terapeutical effect of probiotics in active Crohn's disease and setudy of the mechanism of action. A random, double blind, prospective pilot sttudy comparing enteral nutrition plus placebo during 4 weeks.
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Funding:
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Fundació “La Marató de TV3”
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Referencia:
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Period,:
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from:
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Mar 2004
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to
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Mar 2007
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Project:
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Grupos Consolidados de la Generalitat de Catalunya: “Microbiología de la Enfermedad Inflamatoria Intestinal”
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Year:
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2005
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Funding:
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IV Pla de Recerca. Generalitat de Catalunya
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Role:
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Investigador principal.
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Project:
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Study of microbiota assoiciated to Inflammatory Bowel Diseases and selection of specific bacterial markers for and possible application to support diagnostics in Crohn's Disease and Ulcerative Colitis.
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Reference
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SAF2006-00414
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Year:
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2007-2009
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Funding:
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Plan Nacional de I+D. CICYT
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Role:
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Superfvisor.
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Congressos:
1. Rodriguez-Lazaro, D., X. Fuentes-Garcia, X. Aldeguer, F. Gonzalez-Huix, D. Acero, M. Pla, L.J. Garcia-Gil. Mycobacterium avium sbsp. paratuberculosis was undetectable in. Crohn’s Disease patients using a novel highly specific and sensitive real-time PCR assay. POSTER. XII United European Digestive Week. GUT. Praga; 24 a 29 de Septiembre 2004.
2. L.J. Garcia-Gil, X. Fuentes-Garcia, X. Aldeguer, M. Martinez, F. Gonzalez-Huix
New insights in the microbiology of Crohn’s disease: Classical and emergent bacteria found intimately associated to the intestinal mucosa. POSTER. XII United European Digestive Week GUT. Praga; 24 a 29 de Septiembre 2004.
3. Margarita Martinez-Medina, Xavier Aldeguer, Ferran González-Huix i L. Jesús García-Gil. Diversidad molecular de las bacterias íntimamente asociadas a la mucosa intestinal: en busca de marcadores microbianos de la enfermedad de Crohn.. COMUNICACIÓ ORAL. XIV ReunióN nacional del GETECCU. Madrid; 15 I 16 Octubre de 2004
4. Margarita Martínez-Medina, Xavier Aldeguer, Ferran González-Huix, L. Jesús García-Gil. Anàlisi molecular dels bacteris íntimament associats a la mucosa intestinal de malalts de Crohn. COMUNICACIÓN ORAL. XIV Congrés de la Societat Catalana de Digestologia. Roses (Girona); 27 a 29 de gener de 2005
5. Margarita Martínez, Xavier Aldeguer Manté, Ferran González-Huix Lladó, Jesús Garcia-Gil. Análisis molecular comparado de las bacterias íntimamente asociadas a la mucosa en pacientes con enfermedad de crohn i colitis ulcerosa. POSTER. Reunión Nacional de la Asociación Española de Gastroenterología. Madrid; 10-12 marzo 2005
6. Margarita Martinez-Medina, Xavier Aldeguer, Ferran González-Huix, L. Jesús García-Gil,. High prevalence of Escherichia coli intimately associated to the colonic mucosa of Crohn’s disease patients. POSTER. First International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2005). Badajoz; 15-18 marzo de 2005
7. Laia Calvó, Martí Cortey, Jose-Luís García-Marín, L.Jesús Garcia-Gil. Polygenic analysis of ammonia oxidizing bacteria strains using 16SrDNA, amoA and amoB. POSTER
First International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2005). Badajoz; 15-18 marzo de 2005
8. A. Nadal, A. Coll, N. Thienpont, M. Martínez, F. González-Huix, X. Aldeguer, J. Garcia-Gil and M. Pla.. Quantitative detection of four probiotic lactic acid bacteria by real-time PCR. Monitoring of four probiotic lactic acid bacteria by real-time PCR for clinical purposes. POSTER. First International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2005). Badajoz; 15-18 marzo de 2005
9. L. Jesús Garcia-Gil. Microbiología del Colon Humano. PONENCIA INVITADA
RECAM 2005 . Barcelona; 19 i 20 de maig de 2005.
10. Margarita Martínez-Medina, Xavier Aldeguer, Ferran González-Huix and L. Jesús García-Gil. Mucosal microbiota from healthy and Crohn’s Disease specimens display different fingerprints as revealed by PCR-DGGE.. POSTER. Inflammatory Bowel Disease: Genetic, Barrier Function, Immunologic & microbial pathways. Münster (Alemania); 2-3 Septiembre 2004
11. Margarita Martinez, Xavier Aldeguer, Ferran Gonzalez-Huix, L. Jesús Garcia-Gil
High prevalence of Escherichia coli in intestinal mucosa of Crohn's Disease patients as compared to healthy controls. COMUNICACIÓN ORAL / POSTER. Conference on New Frontiers in Microbial Infection. First Conference: Lessons on Escherichia coli. From Basic research to clinical aspects. Villars Sür Ollon (Suiza); 4 a 8 de Septiembre de 2005
12. Núria Poblet, David Rodríguez-Lázaro, Jordi de Batlle, Javier Ayuso i L. Jesús Garcia-Gil. Detección i análisis de la huella molecular de Mycobacterium spp. mediante PCR-DGGE.. POSTER. XX Congreso de la Sociedad Española de Microbiología
Cáceres; 19-22 de Sept. de 2005
13. De la Torre, M.; J.M. Sirvent, L.J. Garcia-Gil, N. Poblet, B. Quesada i A. Bonet. Estudio prospectivo de una cohorte de pacientes con neumonia adquirida en la comunidad grave y comparación con estudio multicéntrico estatal. COMUNICACIÓN ORAL. SEMICYU 2006
Members
The research group on Clinical Microbiology and Infectious diseases was created in 2004 after a cooperation research project together with the gastroenterologists of the Hospital Josep Trueta of Girona. The main goal of that collaboration was to study the bacterial composition of the colon from Crohn's Disease patients. We have also worked together with the laboratory of Microbiology of that Hospital to investigate the presence of Mycobacterium in the biopsies from those patients. Later, we started to cooperate with the Intensive Care Unit on two programs: one to detect Legionella pneumophila in patients admitted in this unit with severe community acquired pneumonia and the other to study the microbial ecology of the sepsis. After this time working with different aspects of bacterial infections, we are currently focusing our research interest in the Microbial Ecology of the Infection. Our hypothesis is that infections are not monospecific, but they could harbor novel bacteria.
Research lines:
Research subjects:
1. Microbial Ecology of the bacterial Infection
2. Microbiology of the Inflammatory Bowel Disease
Publications:
1. Martinez-Medina, M.; X. Aldeguer, F. Gonzàlez-Huix, L.J. Garcia-Gil. 2006. Abnormal microbiota composition in the ileo-colonic mucosa of Crohn’s disease patients as revealed by PCR-DGGE. Inflammatory Bowel Diseases 12:1136-1145
1.- Rodriguez-Lázaro, D., et al., Mycobacterium avium subsp. paratuberculosis not found in fresh ileocolonic mucosal biopsy specimens from patients with Crohn’s Disease using real-time PCR. Enviado a Microbial Pathogenesis.
Projects:
|
Project:
|
Terapeutical effect of probiotics in active Crohn's disease and setudy of the mechanism of action. A random, double blind, prospective pilot sttudy comparing enteral nutrition plus placebo during 4 weeks.
|
|
Funding:
|
Fundació “La Marató de TV3”
|
Referencia:
|
|
|
Period,:
|
from:
|
Mar 2004
|
to
|
Mar 2007
|
|
Project:
|
Grupos Consolidados de la Generalitat de Catalunya: “Microbiología de la Enfermedad Inflamatoria Intestinal”
|
|
Year:
|
2005
|
|
Funding:
|
IV Pla de Recerca. Generalitat de Catalunya
|
|
Role:
|
Investigador principal.
|
|
Project:
|
Study of microbiota assoiciated to Inflammatory Bowel Diseases and selection of specific bacterial markers for and possible application to support diagnostics in Crohn's Disease and Ulcerative Colitis.
|
|
Reference
|
SAF2006-00414
|
|
Year:
|
2007-2009
|
|
Funding:
|
Plan Nacional de I+D. CICYT
|
|
Role:
|
Superfvisor.
|
Congressos:
1. Rodriguez-Lazaro, D., X. Fuentes-Garcia, X. Aldeguer, F. Gonzalez-Huix, D. Acero, M. Pla, L.J. Garcia-Gil. Mycobacterium avium sbsp. paratuberculosis was undetectable in. Crohn’s Disease patients using a novel highly specific and sensitive real-time PCR assay. POSTER. XII United European Digestive Week. GUT. Praga; 24 a 29 de Septiembre 2004.
2. L.J. Garcia-Gil, X. Fuentes-Garcia, X. Aldeguer, M. Martinez, F. Gonzalez-Huix
New insights in the microbiology of Crohn’s disease: Classical and emergent bacteria found intimately associated to the intestinal mucosa. POSTER. XII United European Digestive Week GUT. Praga; 24 a 29 de Septiembre 2004.
3. Margarita Martinez-Medina, Xavier Aldeguer, Ferran González-Huix i L. Jesús García-Gil. Diversidad molecular de las bacterias íntimamente asociadas a la mucosa intestinal: en busca de marcadores microbianos de la enfermedad de Crohn.. COMUNICACIÓ ORAL. XIV ReunióN nacional del GETECCU. Madrid; 15 I 16 Octubre de 2004
4. Margarita Martínez-Medina, Xavier Aldeguer, Ferran González-Huix, L. Jesús García-Gil. Anàlisi molecular dels bacteris íntimament associats a la mucosa intestinal de malalts de Crohn. COMUNICACIÓN ORAL. XIV Congrés de la Societat Catalana de Digestologia. Roses (Girona); 27 a 29 de gener de 2005
5. Margarita Martínez, Xavier Aldeguer Manté, Ferran González-Huix Lladó, Jesús Garcia-Gil. Análisis molecular comparado de las bacterias íntimamente asociadas a la mucosa en pacientes con enfermedad de crohn i colitis ulcerosa. POSTER. Reunión Nacional de la Asociación Española de Gastroenterología. Madrid; 10-12 marzo 2005
6. Margarita Martinez-Medina, Xavier Aldeguer, Ferran González-Huix, L. Jesús García-Gil,. High prevalence of Escherichia coli intimately associated to the colonic mucosa of Crohn’s disease patients. POSTER. First International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2005). Badajoz; 15-18 marzo de 2005
7. Laia Calvó, Martí Cortey, Jose-Luís García-Marín, L.Jesús Garcia-Gil. Polygenic analysis of ammonia oxidizing bacteria strains using 16SrDNA, amoA and amoB. POSTER
First International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2005). Badajoz; 15-18 marzo de 2005
8. A. Nadal, A. Coll, N. Thienpont, M. Martínez, F. González-Huix, X. Aldeguer, J. Garcia-Gil and M. Pla.. Quantitative detection of four probiotic lactic acid bacteria by real-time PCR. Monitoring of four probiotic lactic acid bacteria by real-time PCR for clinical purposes. POSTER. First International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2005). Badajoz; 15-18 marzo de 2005
9. L. Jesús Garcia-Gil. Microbiología del Colon Humano. PONENCIA INVITADA
RECAM 2005 . Barcelona; 19 i 20 de maig de 2005.
10. Margarita Martínez-Medina, Xavier Aldeguer, Ferran González-Huix and L. Jesús García-Gil. Mucosal microbiota from healthy and Crohn’s Disease specimens display different fingerprints as revealed by PCR-DGGE.. POSTER. Inflammatory Bowel Disease: Genetic, Barrier Function, Immunologic & microbial pathways. Münster (Alemania); 2-3 Septiembre 2004
11. Margarita Martinez, Xavier Aldeguer, Ferran Gonzalez-Huix, L. Jesús Garcia-Gil
High prevalence of Escherichia coli in intestinal mucosa of Crohn's Disease patients as compared to healthy controls. COMUNICACIÓN ORAL / POSTER. Conference on New Frontiers in Microbial Infection. First Conference: Lessons on Escherichia coli. From Basic research to clinical aspects. Villars Sür Ollon (Suiza); 4 a 8 de Septiembre de 2005
12. Núria Poblet, David Rodríguez-Lázaro, Jordi de Batlle, Javier Ayuso i L. Jesús Garcia-Gil. Detección i análisis de la huella molecular de Mycobacterium spp. mediante PCR-DGGE.. POSTER. XX Congreso de la Sociedad Española de Microbiología
Cáceres; 19-22 de Sept. de 2005
13. De la Torre, M.; J.M. Sirvent, L.J. Garcia-Gil, N. Poblet, B. Quesada i A. Bonet. Estudio prospectivo de una cohorte de pacientes con neumonia adquirida en la comunidad grave y comparación con estudio multicéntrico estatal. COMUNICACIÓN ORAL. SEMICYU 2006
Members