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Research Technical Services

Lyophilisation

Lyophilization is the process of sublimation of the water in a sample (liquid to gas transition) from the freezing of a sample and subsequent vacuum drying.

Job request

In order to carry out one analyses, put in touch with the responsible for the UEA.

It be necessary to inform of the toxicity and precautions of storage of the sample.

The equipment

Lyophilisation Telstar LyoQuest-55

Year:
2010



Characteristics:

The LyoQuest-55 is a compact and easy-to-install lyophiliser. 

It has a capacity of 8 Kg of condenser (5 Kg/24 h), with a final temperature of -55 ºC.

The equipment is complemented by a double-stage vacuum pump located outside.

How does it work?

The foundation of the lyophilisation equipment is based on maintaining a previously frozen sample at a low temperature (-50ºC) while the vacuum is applied in a chamber. In this way, the sublimation of the water contained in this sample is obtained. The process ends when the internal temperature of the sample and the chamber are equal (between 24-48h depending on the thickness of the sample). The more surface area of the sample to be lyophilised and the less amount of supernatant, the faster the process will be.

The aim of lyophilisation is to dehydrate samples without altering their composition. Lyophilised samples can be stored in cool, dry places for a long period of time, avoiding their degradation.

Applications / Practical cases

  • The lyophilisation can apply to any solid sample that contains water in its composition (foods, muds, cells, etc).
  • In mixtures of solid and water, the supernatant must be allowed to settle and decanted to shorten the lyophilisation process.
  • It is necessary to avoid, as far as possible, the lyophilisation of samples containing oils and other corrosive chemical products, as they could damage the equipment.

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