Castro, J.; Bravo, M.; Albertí, M.; Marsal, A.; Alonso-De Gennaro, M.J.; Martínez-Ferraté, O.; Claver, C.; Van Leeuwen, P. W. N. M.; Romero, I.; Benito, A.; Vilanova, M. "Dinuclear iron complexes of iminopyridine based ligands as selective cytotoxins for tumor cells and inhibitors of cancer cell migration." Pharmaceutics 14 (2022): 2801-. Jessica Castro; Giusy Tornillo; Gerardo Ceada; Beatriz Ramos-Neble; Marlon Bravo; Marc Ribó; María Vilanova; Matthew J. Smalley; and Antoni Benito "A Nuclear-Directed Ribonuclease Variant Targets Cancer Stem Cells and Inhibits Migration and Invasion of Breast Cancer Cells." Cancers 13 (2021): 4350-. Glòria García-Galindo; Jessica Castro; Jesús Matés; Marlon Bravo; Marc Ribó; Maria Vilanova; Antoni Benito "The Selectivity for Tumor Cells of Nuclear-Directed Cytotoxic RNases Is Mediated by the Nuclear/Cytoplasmic Distribution of p27KIP1." Molecules 26 (2021): 1319-. David Roura Padrosa; Jessica Castro; Alejandro Romero-Casañas; Marc Ribó; Maria Vilanova; Antoni Benito "Construction of Highly Stable Cytotoxic Nuclear-Directed Ribonucleases." Molecules 23 (2018): 3273-. Gordo V; Aparicio D; Pérez-Luque R; Benito A; Vilanova M; Usón I; Fita I; Ribó M "Structural insights into subunits assembly and the oxyester splicing mechanism of neq pol split intein." Cell Chemical Biology 25 (2018): 871-879. Jessica Castro; Ester Manrique; Marlon Bravo; Maria Vilanova; Antoni Benito; Xavier Fontrodona; Montserrat Rodríguez; Isabel Romero "A family of manganese complexes containing heterocyclic-based ligands with cytotoxic properties." Journal of Inorganic Biochemistry 182 (2018): 124-132. A.Vert; J.Castro; M.Ribó; M.Vilanova; A. Benito "Transcriptional profiling of NCI/ADR-RES cells unveils a complex network of signaling pathways and molecular mechanisms of drug resistance." OncoTargets and Therapy 2018 (2018): 221-237. Santiago Ruiz-Martínez; Jessica Castro; Maria Vilanova; Marta Bruix; Douglas V. Laurents; Marc Ribó; Antoni Benito "A Truncated Apoptin Protein Variant Selectively Kills Cancer Cells." Investigational New Drugs 35 (2017): 260-268. Ruiz-Martínez, S.; Pantoja-Uceda, D.; Castro, J.; Vilanova, M.; Ribó, M.; Bruix, M.; Benito, A.; Laurents, V.D. "Insights into the mechanism of Apoptin's exquisitely selective anti-tumor action from atomic level characterization of its conformation and dynamics." Archives of Biochemistry and Biophysics 614 (2017): 53-64. Vert Company, Anna; Castro Gallegos, Jessica; Ribó i Panosa, Marc; Benito i Mundet, Antoni; Vilanova i Brugués, Maria "Activating transcription factor 3 is crucial for antitumor activity and to strengthen the antiviral properties of onconase." Oncotarget (2016): -. Vert Company, Anna; Castro Gallegos, Jessica; Ribó i Panosa, Marc; Benito i Mundet, Antoni; Vilanova i Brugués, Maria "A nuclear-directed human pancreatic ribonuclease (PE5) targets the metabolic phenotype of cancer cells." Oncotarget 7 (2016): 18309-18324. Kurpiewska, K; Dziubek, K; Katrusiak, A; Font, J; Ribò, M; Vilanova, M; Lewiński, K. "Structural investigation of ribonuclease A conformational preferences using high pressure protein crystallography." Chemical Physics 468 (2016): 53-62. Kurpiewska, K.; Torrent, G.; Ribó, M.; Loch, J.I.; Vilanova, M.; Lewiński, K. "Investigating the effects of double mutation C30A/C75A on onconase structure: Studies at atomic resolution." Biopolymers (títol associat amb Peptide Science) 101 (2015): 454-460. Serrano, S., Callís, M., Vilanova, M., Benito, A., Laurents, D.V., Ribó, M. & Bruix, M. "1H, 13C and 15N resonance assignments of the Onconase FL-G zymogen." Biomolecular Nmr Assignments 7 (2013): 13-15. Callís, M., Serrano, S., Benito, A., Laurents, Vilanova, M., D.V., Bruix, M.& Ribó, M. "Towards Tricking a Pathogen’s Protease into Fighting Infection: The 3D Structure of a Stable Circularly Permuted Onconase Variant Cleavedby HIV-1 Protease." PLoS One 8 (2013): e54568-e54568. Vert, A.; Castro, J.; Ruiz-Martínez, S.; Tubert, P., Escribano, D.; Ribó, M.; Vilanova, M.; and Benito, A. "Generation of New Cytotoxic Human Ribonuclease Variants Directed to the Nucleus." Molecular Pharmaceutics 9 (2012): 2894-2902. Castro, Jessica; Ribó, Marc; Puig, Teresa, Colomer, Ramon, Vilanova, Maria & Benito, Antoni "A cytotoxic ribonuclease reduces the expression level of P-glycoprotein in multidrug-resistant cell lines." Investigational New Drugs 30 (2012): 880-888. Tubert, P., Laurents, D.V., Ribó, M., Bruix, M., Vilanova, M. & Benito, A. "Interactions crucial for 3D swapping in the HP-RNase variant PM8." Biophysical Journal 101 (2011): 459-467. Jessica Castro, Marc Ribó, Susanna Navarro, Maria Victòria Nogués, Maria Vilanova and Antoni Benito "A human ribonuclease induces apoptosis associated with p21WAF1/CIP1 induction and JNK inactivation." BMC Cancer 11 (2011): 9-. Ribó, M., Benito, A., Vilanova, M. "Antitumor ribonucleases." Nucleic Acids and Molecular Biology 26 (2011): 55-88. Tubert, Pere; Rodríguez, Montserrat; Ribó, Marc; Benito, Antoni; Vilanova, Maria "The nuclear transport capacity of a human-pancreatic ribonuclease variant is critical for its cytotoxicity." Investigational New Drugs 29 (2011): 811-817. Ercole, Carmine; López Alonso, Jorge Pedro; Font, Josep; Ribó, Marc; Vilanova, Maria; Picone, Delia; Laurents, Douglas V. "Crowding agents and osmolytes provide insight into the formation and dissociation of RNase A oligomers." Archives of Biochemistry and Biophysics 506 (2011): 123-129. López Alonso, Jorge; Bruix, Marta; Font, Josep; Ribó, Marc; Vilanova, Maria; Jimenez, M. Angeles; Santoro, Jorge; González, Carlos; Laurents, Douglas "NMR Spectroscopy Reveals that RNase A is Chiefly Denatured in 40% Acetic Acid: Implications for Oligomer Formation by 3D Domain Swapping." Journal of the American Chemical Society 132 (2010): 1621-1630. López-Alonso, J.P.; Diez-García, F.; Font, J.; Ribó, M.; Vilanova, M.; Scholtz, J.M.; González, C.; Vottariello, F.; Gotte, G.; Libonati, M.; Laurents, D.V. "Carbodiimide EDC induces cross-links that stabilize RNAse A C-dimer against dissociation: EDC adducts can affect protein net charge, conformation and activity." Bioconjugate Chemistry 20 (2009): 1459-1473. Vilà, R.; Benito, A.; Ribó, M.; Vilanova, M. "Mapping the stability clusters in bovine pancreatic ribonuclease A ." Biopolymers (títol associat amb Peptide Science) 91 (2009): 1038-1047. Marchal, S.; Font, J.; Ribó, M.; Vilanova, M.; Phillips, R.; Lange, R.; Torrent, J. "Asymmetric kinetics of protein structural changes." Accounts of Chemical Research 42 (2009): 778-787. Kurpiewska, K.; Font, J.; Ribó, M.; Vilanova, M.; Lewinski, K. "X-ray crystallographic studies of RNase A variants engineered at the most destabilizing positions of the main hydrophobic core: Further insight into protein stability." Proteins 77 (2009): 658-669. Torrent, G.; Ribó, M.; Benito, A.; Vilanova, M. "Bactericidal activity engineered on human pancreatic ribonuclease and onconase." Molecular Pharmaceutics 6 (2009): 531-542. Benito, A.; Laurents, D.V.; Ribó, M.; Vilanova, M. "The structural determinants that lead to the formation of particular oligomeric structures in the pancreatic-type ribonuclease family." Current Protein and Peptide Science 9 (2008): 370-393. Bruix, M.; Ribó, M.; Benito, A.; Laurents, D.V.; Rico, M.; Vilanova, M. "Destabilizing mutations alter the hydrogen exchange mechanism of RNase A." Biophysical Journal 94 (2008): 2297-2305. Rodríguez, M.; Moussaoui, M.; Benito, A.; Cuchillo, C.M.; Nogués, M.V.; Vilanova, M. "Human pancreatic ribonuclease presents higher endonucleolytic activity than ribonuclease A." Archives of Biochemistry and Biophysics 471 (2008): 191-197. Torrent, J.; Marchal, S.; Ribó, M.; Vilanova, M.; Georges, C.; Dupont, Y.; Lange, R. "Distinct unfolding and refolding pathways of ribonuclease A revealed by heating and cooling temperature jumps ." Biophysical Journal 94 (2008): 4056-4065. Benito, A.; Vilanova, M.; Ribó, M. "Intracellular routing of cytotoxic pancreatic-type ribonucleases ." Current Pharmaceutical Biotechnology 9 (2008): 169-179. Torrent, G.; Benito, A.; Castro, J.; Ribó, M.; Vilanova, M. "Contribution of the C30/C75 disulfide bond to the biological properties of onconase." Biological Chemistry Hoppe-Seyler 389 (2008): 1127-1136. Rodríguez, M.; Torrent, G.; Bosch, M.; Rayne, F.; Dubremetz, J.F.; Ribó, M.; Benito, A.; Vilanova, M.; Beaumelle, B. "Intracellular pathway of onconase that enables its delivery to the cytosol." Journal of Cell Science 120 (2007): 1405-1411. Ribó, M.; Font, J.; Benito, A.; Torrent. J.; Lange, R.; Vilanova, M. "Pressure as a tool to study protein-unfolding/refolding processes: the case of ribonuclease A." Biochimica et Biophysica Acta 1764 (2006): 461-469. Rodríguez, M.; Benito, A.; Ribó, M.; Vilanova, M. "Characterization of the dimerization process of a domain-swapped dimeric variant of human pancreatic ribonuclease." Febs Journal 273 (2006): 1166-1176. Font, J.; Torrent, J.; Ribó, M.; Laurents, D.V.; Balny, C.; Vilanova, M.; Lange, R. "Pressure-jump-induced kinetics reveals a hydration dependent folding/unfolding mechanism of ribonuclease A." Biophysical Journal 91 (2006): 2264-2274. Font, J.; Benito, A.; Torrent, J.; Lange, R.; Ribó, M.; Vilanova, M. "Pressure- and temperature-induced unfolding studies:Thermodynamics of core hydrophobicity and packing of ribonuclease A." Biological Chemistry Hoppe-Seyler 387 (2006): 285-296. Font, J.; Benito, A.; Lange, R.; Ribó, M.; Vilanova, M. "The contribution of the residues from the main hydrophobic core of ribonuclease A to its pressure-folding transition state.." Protein Science 15 (2006): 1000-1009. Torrent, J.; Font, J.; Herberhold, H.; Marchal, S.; Ribó, M.; Ruan, K.; Winter, R.; Vilanova, M; Lange, R. "The use of pressure-jump relaxation kinetics to study protein folding landscapes." Biochimica et Biophysica Acta 1764 (2006): 489-496. López-Alonso, J.P.; Bruix, M.; Font, J.; Ribó, M.; Vilanova, M.; Rico, M.; Gotte, G.; Libonati, M.; González, C.; Laurents, D.V. "Formation, structure, and dissociation of the ribonuclease S three-dimensional domain-swapped dimer." Journal of Biological Chemistry 281 (2006): 9400-9406. Rodríguez, M.; Benito, A.; Tubert, P.; Castro, J.; Ribó, M.; Beaumelle, B.; Vilanova, M. "A cytotoxic ribonuclease variant with a discontinuous nuclear localization signal constituted by basic residues scattered over three areas of the molecule." Journal of Molecular Biology 360 (2006): 548-557. Nikolovski, Z.; Buzón, V.; Ribó, M.; Moussaoui, M.; Vilanova, M.; Cuchillo, C.M.; Cladera, J.; Nogués, M.V. "Thermal unfolding of eosinophil cationic protein/ribonuclease 3: a nonreversible process." Protein Science 15 (2006): 2816-2827. Benito, A.; Ribó, M.; Vilanova, M. "On the track of antitumor ribonucleases." Molecular Biosystems 1 (2005): 294-302. Bosch, M.; Benito, A.; Ribó, M.; Puig, T.; Beaumelle, B.; Vilanova, M. "A nuclear localization sequence endows human pancreatic ribonuclease with cytotoxic activity." Biochemistry 43 (2004): 2167-2177. Ribó, M.; Bosch, M.; Torrent, G.; Benito, A.; Beaumelle, B.; Vilanova, M. "Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation process of onconase." European Journal of Biochemistry 271 (2004): 1163-1171. Benito, A.; Bosch, M.; Torrent, G.; Ribó, M.; Vilanova, M. "Stabilisation of human pancreatic ribonuclease through mutation at its N-terminal edge." Protein Engineering 15 (2002): 887-893. Ribó, M.; Benito, A.; Canals, A.; Nogués, M.V.; Cuchillo, C.M.; Vilanova, M. "Purification of engineered human pancreatic ribonuclease." Methods in Enzymology 341 (2001): 221-233. Torrent, J.; Rubens, P.; Ribó, M.; Heremans, K.; Vilanova, M. "Pressure versus temperature-unfolding of ribonuclease A: an FTIR spectroscopic characterization of 10 variants at the carboxy terminal site." Protein Science 10 (2001): 725-734. Canals, A.; Pous, J.; Guasch, A.; Benito, A.; Ribó, M.; Vilanova, M.; Coll, M. "The structure of an engineered domain-swapped ribonuclease dimer and its implications for the evolution of proteins towards oligomerization." Structure 9 (2001): 967-976. Pous, J.; Canals, A.; Terzyan, S.S.; Guasch, A.; Benito, A.; Ribó, M.; Vilanova, M.; Coll, M. "Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the dsign of cytotoxic ribonucleases." Journal of Molecular Biology 303 (2000): 49-59. Torrent, J.; Connelly, J.P.; Rubens, P.; Coll, M.G,; Ribó, M.; Lange, R.; Heremans, K.; Vilanova, M. "The response of the C-terminal variants of ribonuclease A to pressure and temperature-induced unfolding. A study by fourth derivative UV and Fourier transform infrared spectroscopy." High Pressure Research 19 (2000): 247-252. Canals, A., Ribó, M., Benito, A., Bosch, M., Mombelli, E. & Vilanova, M. "Production of engineered human pancreatic ribonucleases, solving expression and purification problems, and enhancing thermostability..." Protein Expression and Purification 17 (1999): 169-181. Coll, M.G., Protasevich, I.I., Torrent, J., Ribó, M., Lobachov, V.M., Makarov, A.A. & Vilanova, M. "Valine 108, a chain folding initiation site belonging residue, crucial for the ribonuclease A stability.." Biochemical and Biophysical Research Communications 265 (1999): 356-360. Torrent, J., Connelly, J.P., Coll, M.G., Ribó, M., Lange, R. & Vilanova, M. "Pressure versus heat-induced unfolding of ribonuclease A: The case of hydrophobic interactions within a chain folding initiation site.." Biochemistry 38 (1999): 15952-15961. Nogués, M.V., Moussaoui, M., Boix, E., Vilanova, M. Ribó, M. & Cuchillo, C.M. "The contribution of noncatalytic phosphate binding subsites to the mechanism of bovine pancreatic ribonuclease A.." Cellular and Molecular Life Sciences 54 (1998): 766-774. Boqué, Ll., Coll, M.G., Ribó, M., Cuchillo, C.M., Fita, I. & Vilanova, M. "Tridimensional structure of three ribonuclease A covalent derivatives with pyridoxal 5'-phosphate.." Protein and Peptide Letters 5 (1998): 101-108. Nogués, M.V., Vilanova, M. & Cuchillo, C.M. "Bovine pancreatic ribonuclease A as a model of an enzyme with multiple substrate binding sites.." Biochimica et Biophysica Acta 1253 (1995): 16-24. Boqué, Ll.,Coll, M.G.,Vilanova, M., Cuchillo, C.M. & Fita, I. "The structure of ribonuclease A derivative II at 2.1.Å resolution.." Journal of Biological Chemistry 269 (1994): 19707-19712. Vendrell, J., Avilés, F.X., Vilanova, M., Turner, C.H. & Crane-Robinson, C. "Proton magnetic resonance studies on the isolated activation segment from porcine procarboxypeptidase.." Biochemical Journal 267 (1990): 231-238. Vilanova, M., Vendrell, J., Cuchillo, C.M. & Avilés, F.X. "Analysis of the conformation and ligand-binding properties of the activation segment of pig procarboxypeptidase A.." Biochemical Journal 252 (1988): 901-905. Sánchez-Ruiz, J.M., López-Lacomba, J.L., Mateo, P.L.,Vilanova, M., Serra, M.A. & Avilés, F.X. "Analysis of the thermal unfolding of porcine procarboxypeptidase A and its tryptic pieces by differential scanning calorimetry.." European Journal of Biochemistry 176 (1988): 225-230. Vilanova, M., Avilés, F.X., Vendrell, J. & Méndez, E. "Conformational predictive studies on the activation segment of pancreatic procaroxypeptidases.." Biochemical and Biophysical Research Communications 149 (1987): 729-734. López, J.L., Mateo, P.L., Cortijo, M., Vilanova, M., Muñoz, A. & Avilés, F.X. "A scanning microcalorimetric study of procarboxypeptidase A and its tryptic pieces, carboxypeptidase A and activation segmemt.." Biochemical Society Transactions 13 (1985): 343-344. Vilanova, M., Vendrell, J., López. M.T., Cuchillo, C.M. & Avilés, F.X. "Preparative isolation of the two forms of pig pancreatic pro-(carboxypeptidase A) and their monomeric carboxypeptidases A.." Biochemical Journal 229 (1985): 605-609. Vilanova, M., Burgos, F.J., Cuchillo, C.M. & Avilés, F.X. "Urea-gradient electrophoresis studies on the association of procarboxypeptidase A and B, proproteinase E, and their tryptic activation products.." Febs Letters 191 (1985): 273-277. San Segundo, B., Martínez, M.C., Vilanova, M., Cuchillo, C.M. & Avilés, F.X. "The severed activation segment of porcine pancreatic procarboxypeptidase A is a powerful inhibitor of the active enzyme. Isolation and characterization of the activation peptide.." Acta Biochimica et Biophysica Hungarica 707 (1982): 74-80. Avilés, F.X., San Segundo, B., Vilanova, M., Cuchillo, C.M. & Turner C. "The activation segment of procarboxypeptidase A from porcine pancreas constitutes a folded structural domain.." Febs Letters 149 (1982): 257-260.
Castro J; Ribo M; Vilanova M, Benito A "Strengths and challenges of secretory ribonucleases as antitumor agents." Pharmaceutics 13 (2021): 1-17. Castro J; Ribo M; Benito A; Vilanova M "Apoptin, a Versatile Protein with Selective Antitumor Activity." Current Medicinal Chemistry (2018): -. Castro J; Ribó M; Benito A; Vilanova M "MiniReview: Nucleus-targeted ribonucleases as antitumor drugs." Current Medicinal Chemistry 20 (2013): 1225-1231.
S. Ruíz-Martínez; J. Castro; M. Vilanova; M. Bruix; M. Ribó; V. D. Laurents; A. Benito "Antitumor Viral protein variant selectively cytotoxic for cancer cells when exogenously added." Febs Journal 282 (2015): 244-. Vilanova, M., Castro, J., Tubert, P., Ribo, M., Benito, A. "Ribonucleases directed to the nucleus as a novel proapoptotic anticancer strategy." New Biotechnology 25 (2009): S8-S9. Rodríguez, M.; Benito, A.; Tubert, P.; Castro, J.; Ribó, M.; Beaumelle, B.; Vilanova, M. "Characterization of a bipartite-equivalent nuclear localization signal of a cytotoxic ribonuclease variant." Febs Journal 273 (2006): 290-290.