J Castro; A Benito; M Vilanova; M Ribó; G Tornillo; MJ Smalley "A nuclear-directed human pancreatic ribonuclease variant is cytotoxic for breast cancer cells cultured in 3D and kills cancer stem cells." Esmo Open 3 (2018): 190-. S. Ruíz-Martínez; J. Castro; M. Vilanova; M. Bruix; M. Ribó; V. D. Laurents; A. Benito "Antitumor Viral protein variant selectively cytotoxic for cancer cells when exogenously added." Febs Journal 282 (2015): 244-. Vilanova, M., Castro, J., Tubert, P., Ribo, M., Benito, A. "Ribonucleases directed to the nucleus as a novel proapoptotic anticancer strategy." New Biotechnology 25 (2009): S8-S9. Rodríguez, M.; Benito, A.; Tubert, P.; Castro, J.; Ribó, M.; Beaumelle, B.; Vilanova, M. "Characterization of a bipartite-equivalent nuclear localization signal of a cytotoxic ribonuclease variant." Febs Journal 273 (2006): 290-290.
Castro, J.; Bravo, M.; Albertí, M.; Marsal, A.; Alonso-De Gennaro, M.J.; Martínez-Ferraté, O.; Claver, C.; Van Leeuwen, P. W. N. M.; Romero, I.; Benito, A.; Vilanova, M. "Dinuclear iron complexes of iminopyridine based ligands as selective cytotoxins for tumor cells and inhibitors of cancer cell migration." Pharmaceutics 14 (2022): 2801-. Roger Fernández, Aida Carreño, Rosa Mendoza, Antoni Benito, Neus Ferrer-Miralles, María Virtudes Céspedes, José Luis Corchero "Escherichia coli as a New Platform for the Fast Production of Vault-like Nanoparticles: An Optimized Protocol." International Journal of Molecular Sciences 23 (2022): 15543-. Fernando Martín; Aida Carreño; Rosa Mendoza; Pablo Caruana; Francisco Rodriguez; Marlon Bravo; Antoni Benito; Neus Ferrer-Miralles; M Virtudes Céspedes; José Luis Corchero "All-in-one biofabrication and loading of recombinant vaults in human cells." Biofabrication 14 (2022): -. Jessica Castro; Giusy Tornillo; Gerardo Ceada; Beatriz Ramos-Neble; Marlon Bravo; Marc Ribó; María Vilanova; Matthew J. Smalley; and Antoni Benito "A Nuclear-Directed Ribonuclease Variant Targets Cancer Stem Cells and Inhibits Migration and Invasion of Breast Cancer Cells." Cancers 13 (2021): 4350-. Glòria García-Galindo; Jessica Castro; Jesús Matés; Marlon Bravo; Marc Ribó; Maria Vilanova; Antoni Benito "The Selectivity for Tumor Cells of Nuclear-Directed Cytotoxic RNases Is Mediated by the Nuclear/Cytoplasmic Distribution of p27KIP1." Molecules 26 (2021): 1319-. David Roura Padrosa; Jessica Castro; Alejandro Romero-Casañas; Marc Ribó; Maria Vilanova; Antoni Benito "Construction of Highly Stable Cytotoxic Nuclear-Directed Ribonucleases." Molecules 23 (2018): 3273-. Gordo V; Aparicio D; Pérez-Luque R; Benito A; Vilanova M; Usón I; Fita I; Ribó M "Structural insights into subunits assembly and the oxyester splicing mechanism of neq pol split intein." Cell Chemical Biology 25 (2018): 871-879. Jessica Castro; Ester Manrique; Marlon Bravo; Maria Vilanova; Antoni Benito; Xavier Fontrodona; Montserrat Rodríguez; Isabel Romero "A family of manganese complexes containing heterocyclic-based ligands with cytotoxic properties." Journal of Inorganic Biochemistry 182 (2018): 124-132. A.Vert; J.Castro; M.Ribó; M.Vilanova; A. Benito "Transcriptional profiling of NCI/ADR-RES cells unveils a complex network of signaling pathways and molecular mechanisms of drug resistance." OncoTargets and Therapy 2018 (2018): 221-237. Santiago Ruiz-Martínez; Jessica Castro; Maria Vilanova; Marta Bruix; Douglas V. Laurents; Marc Ribó; Antoni Benito "A Truncated Apoptin Protein Variant Selectively Kills Cancer Cells." Investigational New Drugs 35 (2017): 260-268. Ruiz-Martínez, S.; Pantoja-Uceda, D.; Castro, J.; Vilanova, M.; Ribó, M.; Bruix, M.; Benito, A.; Laurents, V.D. "Insights into the mechanism of Apoptin's exquisitely selective anti-tumor action from atomic level characterization of its conformation and dynamics." Archives of Biochemistry and Biophysics 614 (2017): 53-64. Vert Company, Anna; Castro Gallegos, Jessica; Ribó i Panosa, Marc; Benito i Mundet, Antoni; Vilanova i Brugués, Maria "Activating transcription factor 3 is crucial for antitumor activity and to strengthen the antiviral properties of onconase." Oncotarget (2016): -. Vert Company, Anna; Castro Gallegos, Jessica; Ribó i Panosa, Marc; Benito i Mundet, Antoni; Vilanova i Brugués, Maria "A nuclear-directed human pancreatic ribonuclease (PE5) targets the metabolic phenotype of cancer cells." Oncotarget 7 (2016): 18309-18324. J. Castro, M. Ribo, A. Benito and M. Vilanova . "Nucleus-Targeted Ribonucleases As Antitumor Drugs." Current Medicinal Chemistry 20 (2013): 1225-1231. Serrano, S., Callís, M., Vilanova, M., Benito, A., Laurents, D.V., Ribó, M. & Bruix, M. "1H, 13C and 15N resonance assignments of the Onconase FL-G zymogen." Biomolecular Nmr Assignments 7 (2013): 13-15. Callís, M., Serrano, S., Benito, A., Laurents, Vilanova, M., D.V., Bruix, M.& Ribó, M. "Towards Tricking a Pathogen’s Protease into Fighting Infection: The 3D Structure of a Stable Circularly Permuted Onconase Variant Cleavedby HIV-1 Protease." PLoS One 8 (2013): e54568-e54568. Vert, A.; Castro, J.; Ruiz-Martínez, S.; Tubert, P., Escribano, D.; Ribó, M.; Vilanova, M.; and Benito, A. "Generation of New Cytotoxic Human Ribonuclease Variants Directed to the Nucleus." Molecular Pharmaceutics 9 (2012): 2894-2902. Castro, Jessica; Ribó, Marc; Puig, Teresa, Colomer, Ramon, Vilanova, Maria & Benito, Antoni "A cytotoxic ribonuclease reduces the expression level of P-glycoprotein in multidrug-resistant cell lines." Investigational New Drugs 30 (2012): 880-888. Tubert, Pere; Rodríguez, Montserrat; Ribó, Marc; Benito, Antoni; Vilanova, Maria "The nuclear transport capacity of a human-pancreatic ribonuclease variant is critical for its cytotoxicity." Investigational New Drugs 29 (2011): 811-817. Tubert, P., Laurents, D.V., Ribó, M., Bruix, M., Vilanova, M. & Benito, A. "Interactions crucial for 3D swapping in the HP-RNase variant PM8." Biophysical Journal 101 (2011): 459-467. Jessica Castro, Marc Ribó, Susanna Navarro, Maria Victòria Nogués, Maria Vilanova and Antoni Benito "A human ribonuclease induces apoptosis associated with p21WAF1/CIP1 induction and JNK inactivation." BMC Cancer 11 (2011): 9-. Ribó, M., Benito, A., Vilanova, M. "Antitumor ribonucleases." Nucleic Acids and Molecular Biology 26 (2011): 55-88. Vilà, R.; Benito, A.; Ribó, M.; Vilanova, M. "Mapping the stability clusters in bovine pancreatic ribonuclease A ." Biopolymers (títol associat amb Peptide Science) 91 (2009): 1038-1047. Torrent, G.; Ribó, M.; Benito, A.; Vilanova, M. "Bactericidal activity engineered on human pancreatic ribonuclease and onconase." Molecular Pharmaceutics 6 (2009): 531-542. Rodríguez, M.; Moussaoui, M.; Benito, A.; Cuchillo, C.M.; Nogués, M.V.; Vilanova, M. "Human pancreatic ribonuclease presents higher endonucleolytic activity than ribonuclease A." Archives of Biochemistry and Biophysics 471 (2008): 191-197. Benito, A.; Vilanova, M.; Ribó, M. "Intracellular routing of cytotoxic pancreatic-type ribonucleases ." Current Pharmaceutical Biotechnology 9 (2008): 169-179. Torrent, G.; Benito, A.; Castro, J.; Ribó, M.; Vilanova, M. "Contribution of the C30/C75 disulfide bond to the biological properties of onconase." Biological Chemistry Hoppe-Seyler 389 (2008): 1127-1136. Benito, A.; Laurents, D.V.; Ribó, M.; Vilanova, M. "The structural determinants that lead to the formation of particular oligomeric structures in the pancreatic-type ribonuclease family." Current Protein and Peptide Science 9 (2008): 370-393. Bruix, M.; Ribó, M.; Benito, A.; Laurents, D.V.; Rico, M.; Vilanova, M. "Destabilizing mutations alter the hydrogen exchange mechanism of RNase A." Biophysical Journal 94 (2008): 2297-2305. Rodríguez, M.; Torrent, G.; Bosch, M.; Rayne, F.; Dubremetz, J.F.; Ribó, M.; Benito, A.; Vilanova, M.; Beaumelle, B. "Intracellular pathway of onconase that enables its delivery to the cytosol." Journal of Cell Science 120 (2007): 1405-1411. Font, J.; Benito, A.; Torrent, J.; Lange, R.; Ribó, M.; Vilanova, M. "Pressure- and temperature-induced unfolding studies:Thermodynamics of core hydrophobicity and packing of ribonuclease A." Biological Chemistry Hoppe-Seyler 387 (2006): 285-296. Ribó, M.; Font, J.; Benito, A.; Torrent. J.; Lange, R.; Vilanova, M. "Pressure as a tool to study protein-unfolding/refolding processes: the case of ribonuclease A." Biochimica et Biophysica Acta 1764 (2006): 461-469. Rodríguez, M.; Benito, A.; Ribó, M.; Vilanova, M. "Characterization of the dimerization process of a domain-swapped dimeric variant of human pancreatic ribonuclease." Febs Journal 273 (2006): 1166-1176. Font, J.; Benito, A.; Lange, R.; Ribó, M.; Vilanova, M. "The contribution of the residues from the main hydrophobic core of ribonuclease A to its pressure-folding transition state.." Protein Science 15 (2006): 1000-1009. Rodríguez, M.; Benito, A.; Tubert, P.; Castro, J.; Ribó, M.; Beaumelle, B.; Vilanova, M. "A cytotoxic ribonuclease variant with a discontinuous nuclear localization signal constituted by basic residues scattered over three areas of the molecule." Journal of Molecular Biology 360 (2006): 548-557. Benito, A.; Ribó, M.; Vilanova, M. "On the track of antitumor ribonucleases." Molecular Biosystems 1 (2005): 294-302. Ribó, M.; Bosch, M.; Torrent, G.; Benito, A.; Beaumelle, B.; Vilanova, M. "Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation process of onconase." European Journal of Biochemistry 271 (2004): 1163-1171. Bosch, M.; Benito, A.; Ribó, M.; Puig, T.; Beaumelle, B.; Vilanova, M. "A nuclear localization sequence endows human pancreatic ribonuclease with cytotoxic activity." Biochemistry 43 (2004): 2167-2177. Benito, A.; Bosch, M.; Torrent, G.; Ribó, M.; Vilanova, M. "Stabilisation of human pancreatic ribonuclease through mutation at its N-terminal edge." Protein Engineering 15 (2002): 887-893. Canals, A.; Pous, J.; Guasch, A.; Benito, A.; Ribó, M.; Vilanova, M.; Coll, M. "The structure of an engineered domain-swapped ribonuclease dimer and its implications for the evolution of proteins towards oligomerization." Structure 9 (2001): 967-976. Ribó, M.; Benito, A.; Canals, A.; Nogués, M.V.; Cuchillo, C.M.; Vilanova, M. "Purification of engineered human pancreatic ribonuclease." Methods in Enzymology 341 (2001): 221-233. Pous, J.; Canals, A.; Terzyan, S.S.; Guasch, A.; Benito, A.; Ribó, M.; Vilanova, M.; Coll, M. "Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the dsign of cytotoxic ribonucleases." Journal of Molecular Biology 303 (2000): 49-59. Canals, A., Ribó, M., Benito, A., Bosch, M., Mombelli, E. & Vilanova, M. "Production of engineered human pancreatic ribonucleases, solving expression and purification problems, and enhancing thermostability..." Protein Expression and Purification 17 (1999): 169-181. Zeder-Lutz, G., Benito, A. y Van Regenmortel, M.H.V. "Active concentration measurements of recombinant biomolecules using biosensor technology.." Journal of Molecular Recognition 12 (1999): 300-309. Feliu, J.X., Benito, A. Oliva, B., Avilés, F.X. y Villaverde, A. "Conformational flexibility in a highly mobile protein loop of foot-and-mouth disease virus: distinct structural requirements for integrin and antibody binding.." Journal of Molecular Biology 283 (1998): 331-338. Carbonell, X., Feliu, J.X., Benito, A. y Villaverde, A. "Display-induced variation in recombinant peptides.." Biochemical and Biophysical Research Communications 248 (1998): 773-777. Benito, A. y Van Regenmortel, M.H.V. "Biosensor characterization of antigenic site A of foot-and-mouth disease virus presented in different vector systems.." Fems Immunology and Medical Microbiology 21 (1998): 101-115. Arís, A., Corchero, J.L., Benito, A., Carbonell, X., Viaplana, E. y Villaverde, A. "The expression of recombinant genes from bacteriophage lambda strong promoters triggers the SOS response in Escherichia coli.." Biotechnology and Bioengineering 60 (1998): 551-559. Villaverde, A., Feliu, J.X., Arís, A., Harbottle, R.P., Benito, A. y Coutelle, C. "A cell adhesion peptide from foot-and-mouth disease virus can direct cell targeted delivery of a functional enzyme.." Biotechnology and Bioengineering 59 (1998): 294-301. Carbonell, X., Benito, A. y Villaverde, A. "Converging antigenic structure of a recombinant viral peptide displayed on different frameworks of carrier proteins.." Febs Letters 397 (1996): 169-172. Villaverde, A., Feliu, J.X. Harbottle, R.P., Benito, A, y Coutelle, C. "A recombinant, arginine-glycine-aspartic acid (RGD) motif from foot-and-mouth disease virus binds mammalian cells through vitronectin and, to a lower extent, fibronectin receptors." Gene 180 (1996): 101-106. Benito, A., Feliu, J.X. y Villaverde, A. "beta-Galactosidase enzymatic activity as a molecular probe to detect specific antibodies.." Journal of Biological Chemistry 271 (1996): 21251-21256. Corchero, J.L., Viaplana, E., Benito, A. y Villaverde, A. "The position of the heterologous domain can influence the solubility and proteolysis of beta-galactosidase fusion proteins in E. coli." Journal of Biotechnology 48 (1996): 191-200. Benito, A., Feliu, J.X. y Villaverde, A. "Peptide insertions in beta-galactosidase activating interface can improve binding in TPEG-Sepharose affinity chromatography.." Biotechnology Techniques 9 (1995): 767-770. Benito, A., Viaplana, E., Corchero, JL., Carbonell, X. y Villaverde, A. "A recombinant foot-and-mouth disease virus antigen inhibits DNA replication and triggers the SOS response in Escherichia coli.." FEMS Microbiology Letters 129 (1995): 157-162. Corchero, J.L., Carbonell, X., Benito, A. y Villaverde, A. "Mitomycin C stimulates thermally induced recombinant gene expression in Escherichia coli MC strains.." Applied Microbiology and Biotechnology 42 (1995): 890-894. Benito, A., Mateu, M.G. y Villaverde, A. "Improved mimicry of a foot-and-mouth disease virus antigenic site by a viral peptide displayed on beta-galactosidase surface.." Bio-Technology 13 (1995): 801-804. Vila, P., Corchero, J.L., Benito, A. y Villaverde A. "Ammonium-mediated reduction of plasmid copy number and recombinant gene expression in Escherichia coli.." Biotechnology Progress 10 (1994): 648-651. Benito, A. y Villaverde, A. "Insertion of a 27 amino acid viral peptide in different zones of Escherichia coli beta-galactosidase: effects on the enzyme activity.." FEMS Microbiology Letters 123 (1994): 107-112. Villaverde, A., Benito, A., Viaplana, E. y Cubarsí, R. "Fine regulation of CI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature.." Applied and Environmental Microbiology 59 (1993): 3485-3487. Benito, A., Vidal, M. y Villaverde, A. "Enhanced production of pL-controlled recombinant proteins and plasmid stability in Escherichia coli Rec A+ strains.." Journal of Biotechnology 29 (1993): 299-306. Benito, A., Valero, F., Lafuente, J., Vidal, M., Cairó, J., Solà, C. y Villaverde, A. "Uses of ß-galactosidase tag in on- line monitoring production of fusion proteins and gene expresion in Escherichia coli.." Enzyme and Microbial Technology 15 (1993): 63-71. Valero, F., Lafuente, F.J., Solà, C., Benito, A., Vidal, M., Cairó, J. y Villaverde, A. "Simultaneous on line monitoring of intracellular ß-galactosidase activity and biomass using flow injection analysis in Escherichia coli batch fermentations.." Biotechnology Techniques 6 (1992): 213-218. Cubarsí, R., Benito, A., Vidal, M., Cairó, J. y Villaverde, A. "A model for continuous production of thermally induced recombinant proteins." Biotechnology Letters 13 (1991): 249-254.
Castro J; Ribo M; Vilanova M, Benito A "Strengths and challenges of secretory ribonucleases as antitumor agents." Pharmaceutics 13 (2021): 1-17. Castro J; Ribo M; Benito A; Vilanova M "Apoptin, a Versatile Protein with Selective Antitumor Activity." Current Medicinal Chemistry (2018): -. Castro J; Ribó M; Benito A; Vilanova M "MiniReview: Nucleus-targeted ribonucleases as antitumor drugs." Current Medicinal Chemistry 20 (2013): 1225-1231.