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14 February 2017
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Teaching is concentrated in the faculties and schools, and the departments deal with research, which is also conducted by institutes and chairs, at the same time responsible for knowledge promotion.
It ionic chromatography it is related with the liquid chromatography of high performance. It is a technique that it allows to detect ionic and strongly polar species. The stationary phase is a liquid phase connected with capacity to exchange ions. The solutes have to be ionic, and its separation is a product of the equilibria of displacement of some ions for others of the same sign. People practice in high pressure.
With this technique, the anàlits present to the sample break away depending on its bachelor's degree of retention when they are put with the stationary phase of the analytical columns in touch. Once sorted out, the sample goes through a detector, in our case, of conductivity or amperomètric, where the signal obtained with respect to the time of retention is registered. Some chromatograms in where the position of the pike indicates us the present ion (qualitative character) are the result and its area indicates us the amount of each ion (quantitative character).
In order to carry out one analyses, put in touch with the responsible for the UEA.
The necessary volume of sample is 2 mL (filtrates in 0.2 µm).
It be necessary to inform of the toxicity and precautions of storage of the sample. Once the report has been given, if people do not come to search the remainder of the sample after a week, this will be destroyed.
The principle of the method is the separation of ions for chromatography in liquid phase through a column of separation. The stationary phase is an ionic exchanger (aniònic or catiònic) and, the mobile phase (eluent) is an aqueous solution in basic environment (for eluir anions or sugars), or in acid environment (for eluir the cations). The used detector is of conductivity in the case of the analysis of anions and/or cations, and amperomètric for the analysis of sugars.
When the detector of conductivity is used, it is essential that the eluents have a conductivity low enough. For this reason, our system combines the detector of conductivity with a system electrochemical suppressor who reduces the conductivity in the background caused by the eluent and increases the conductivity of the anàlits ions. The concentration of the different ions is determined calibrating the procedure in its whole.
The chromatograph can have two configurations: one for the analysis of anions and/or cations, and one for the analysis of sugars.
In all cases, the necessary volume of sample is 2 mL (filtrates in 0.2 µm).
Analysis of anions and cations:
For the analysis of anions and cations, the chromatograph is equipped with two columns: the IonPac AS19 of Thermo Scientific for the analysis of anions, and the Ion Pac CS16 of Thermo Scientific for the analysis of cations. The equipment's configuration allows simultaneous analyses of dissolved cations and anions.
The used method allows the concentration of anions and cations dissolved in liquid samples to be determined. It enables, in particular, the determination of the following anions and cations:
Sugar analysis:
An amperometric detector is used for analysing sugars, equipped with a benchmar pH-Ag/AgCl electrode. The column used is the CarboPac PA20.
INTERCOMPARISON TEST FOR ANIONS AND CATIONS
The participation in this essay is a form of measuring the quality of the results through an external evaluation.
They usually take part in it around 15 laboratories of the international area. It consists of the analysis of two samples of continental waters, giving results of the contents of different anions and/or cations.
The STR take part in it since 2022 bringing the results obtained with the technique of ionic chromatography, always obtaining satisfactory results.
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