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Research Technical Services

Direct optical microscopy and spectroscopy

Based on optic lenses

An optical microscope, whether simple or compound, is a microscope based on optical lenses.

Job request

In order to request that a sample be analysed, you need to contact the staff responsible for the team.

You will need to give the toxicity and any storage precautions. Once the report has been delivered, if you do not collect the sample within one week, any remaining sample will be destroyed.

The equipment

KIOWA OPTICAL SD2PLQ stereoscopic microscope, with Greenough optical paths, (20x/40x) with direct and reflected halogen lighting.

Estereoscòpic microscope NIKON SMZ1000, of parallel optic paths, (8-80x) with systems of direct and oblique transmitted halògena lighting of light, reflected light and polarisation. Has 3.34-megapixel NIKON Coolpix-990 digital camera image recording.

Estereoscòpic microscope ZEISS SteREO Discovery V12, motorised and provided with digital electronic control Zeiss SyCopd'il·luminació, d'enfoc and of increase. Optical system of parallel optical paths and optics corrected to infinite). Trinocular head objective revolver with plan achromatic optics of 0.63x and 1.5x and achromatic of 0.3x. Continuous optical zoom (3-150x), with systems of direct and oblique transmitted cold halogen lighting and reflected zenith and lateral lighting. Incorporates a motorised PRIOR platen. Infinity X of 3,75MPícsels and software of microscope control has register of digital image for digital camera, platen and of acquisition of images DeltaPix Pro.

Motorised LEICA DMR-XA direct biological optical microscope, optics corrected to infinite, (50-1600x), with episcopic and diascopic halogen white lighting systems, and epifluorescence. Allows work in clear field, dark field, contrast of phases, polarisation and interference contrast (DIC). Has systems of photographic image recording with bodies of 135 format NIKON F90 and RICOH X-RX3000 cameras and digital video images with a 3CCD SONY DXC950-P camera.

ZEISS JENAVERT direct metallographic optical microscope, (40-1250x), with episcopic and diascopic white lighting systems. Allows work in clear field, dark field, contrast of phases, polarisation. Has a microhardness tester. Has systems of conventional photographic image recording with body of 135 format RICOH X-RX3000 camera and digital video images with an HITACHI VK_C220E camera.

How does it work?

See equipment features.

Applications / Practical cases

Optical microscopes are used to study thin specimens, since their depth of field is very limited. In general, they are used to examine crops, triturated preparations or a very fine sheet of any material. Normally depends on the light that pierces the sample from below and special techniques are usually needed to increase the image contrast.

More information:

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