Estudia

• 1. Identificar problemes, buscar solucions originals i aplicar-les en un context d'investigació o professional
• 2. Contribuir al teixit europeu d'investigació i desenvolupament amb una visió àmplia i multidisciplinària dins dels camps biològic, biotecnològic, bioquímic, químic i biomèdic
• 4. Comunicar de forma clara i concisa els seus coneixements, les seves conclusions i les implicacions ètiques i socials del seu camp de treball a públics especialitzats i no especialitzats ( divulgació científica)
• 5. Actualitzar i prosseguir els seus estudis de forma auto-dirigida i autònoma, recollint i seleccionant la informació necessària que permeti una investigació original i que aporti nous coneixements
• 11. Identificar i analitzar els mecanismes moleculars bàsics que operen en els éssers vius, particularment l'home, així com les seves aplicacions en diferents camps d'investigació
• 15. Valorar la utilitat de tècniques de modificació genètica d'organismes per a estudis i tractament de patologies humanes, la millora de processos i productes biotecnològics

1. How gene expression regulation takes place in eukaryotes. Chromatin organization.Histones modification: acetylation, phosphorylation, ubiquitination and sumoylation. Chromatin remodeling complexes. DNA methylation. Loops and chromosome territories.

2. How gene expression regulation takes place in eukaryotes and its comparison with the same process in prokaryotes. DNA sequences involved in transcription: promoters, enhancers, silencers (cis elements). Trans-elements: basal transcription factors, transactivators, coactivators, HMG proteins. Gene expression regulation and signal transduction relationship.

3. Gene expression regulation at post/co-transcriptional level: mechanisms involved. Intron removal. Spliceosome assembly. Alternative splicing: types and functions. How the splicing is regulated?

4. Gene silencing: DICER and RISC complexes, Drosha and its function. Proteins involved in this process. Biogenesis of siRNAs and miRNAs: differences. How siRNAs and miRNAs silence gene expression. Gene silencing and chromatin remodeling relationship.

5. Nucleus-cytoplasm transport of proteins and RNAs: regulatory processes of gene expression. RNAs half-life and their degradation.

6. Regulation of gene expression at post-transcriptional level. Protein targeting. Protein post-translational modifications and their functions.

• Allis, C. David (cop. 2007 ). Epigenetics . Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press. Catàleg
• Alberts, Bruce (cop. 2002 ). Molecular biology of the cell (4th ed). New York [etc.]: Garland. Catàleg
• Hannon, Gregory J. (cop. 2003 ). RNAI : a guide to gene silencing . Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory. Catàleg
• Watson, James D. (cop. 2007 ). Recombinant DNA : genes and genomes, a short course (3th ed.). New York: W. H. Freema and Co. Catàleg
• Watson, James D. (2008 ). Molecular biology of the gene (6th ed.). New York: Cold Spring Harbor Laboratory Press [etc.]. Catàleg
• Watson, James D. (2005 ). Biología molecular del gen (5ª ed.). Madrid [etc.]: Médica Panamericana. Catàleg
• Lodish, Harvey F. (cop. 2000 ). Molecular cell biology (4th ed.). New York: W.H. Freeman. Catàleg
• Varki, Ajit (2008 ). The Essentials of glycobiology (2a ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory Press. Catàleg

Activitats d'avaluació:

The final grade of the written work will be the same for all the group members that have been involved in the work preparation.
The grade of the individual final exam will be the sum of each of the exercises proposed. Their value will be indicated in each exercise. To pass the course it is mandatory to reach a minimum of 5 over 10 in this exam.

In case that any student does not get a final grade of 5 over 10, only the individual final exam can be repeated.

Criteris específics de la nota «No Presentat»:
It is mandatory the attendance at a minimum of 80% of the lectures. A NOT PRESENTED student implies that he/she has not done the final control exam or that has not presented the written work.