Vilanova, M., Castro, J., Tubert, P., Ribo, M., Benito, A. "Ribonucleases directed to the nucleus as a novel proapoptotic anticancer strategy." New Biotechnology 25 (2009): S8-S9.
Gordo V; Aparicio D; Pérez-Luque R; Benito A; Vilanova M; Usón I; Fita I; Ribó M "Structural insights into subunits assembly and the oxyester splicing mechanism of neq pol split intein." Cell Chemical Biology 25 (2018): 871-879. A.Vert; J.Castro; M.Ribó; M.Vilanova; A. Benito "Transcriptional profiling of NCI/ADR-RES cells unveils a complex network of signaling pathways and molecular mechanisms of drug resistance." OncoTargets and Therapy 2018 (2018): 221-237. Santiago Ruiz-Martínez; Jessica Castro; Maria Vilanova; Marta Bruix; Douglas V. Laurents; Marc Ribó; Antoni Benito "A Truncated Apoptin Protein Variant Selectively Kills Cancer Cells." Investigational New Drugs 35 (2017): 260-268. Fagagnini A, Montioli R, Caloiu A, Ribó M, Laurents DV, Gotte G. "Extensive deamidation of RNase A inhibits its oligomerization through 3D domain swapping.." Biochimica et Biophysica Acta. Proteins and Proteomics 1865 (2017): 76-87. Vert Company, Anna; Castro Gallegos, Jessica; Ribó i Panosa, Marc; Benito i Mundet, Antoni; Vilanova i Brugués, Maria "Activating transcription factor 3 is crucial for antitumor activity and to strengthen the antiviral properties of onconase." Oncotarget (2016): -. Kurpiewska, K; Dziubek, K; Katrusiak, A; Font, J; Ribò, M; Vilanova, M; Lewiński, K. "Structural investigation of ribonuclease A conformational preferences using high pressure protein crystallography." Chemical Physics 468 (2016): 53-62. Vert Company, Anna; Castro Gallegos, Jessica; Ribó i Panosa, Marc; Benito i Mundet, Antoni; Vilanova i Brugués, Maria "A nuclear-directed human pancreatic ribonuclease (PE5) targets the metabolic phenotype of cancer cells." Oncotarget 7 (2016): 18309-18324. Kurpiewska, K.; Torrent, G.; Ribó, M.; Loch, J.I.; Vilanova, M.; Lewiński, K. "Investigating the effects of double mutation C30A/C75A on onconase structure: Studies at atomic resolution." Biopolymers (títol associat amb Peptide Science) 101 (2015): 454-460. J. Castro, M. Ribo, A. Benito and M. Vilanova . "Nucleus-Targeted Ribonucleases As Antitumor Drugs." Current Medicinal Chemistry 20 (2013): 1225-1231. Serrano, S., Callís, M., Vilanova, M., Benito, A., Laurents, D.V., Ribó, M. & Bruix, M. "1H, 13C and 15N resonance assignments of the Onconase FL-G zymogen." Biomolecular Nmr Assignments 7 (2013): 13-15. Callís, M., Serrano, S., Benito, A., Laurents, Vilanova, M., D.V., Bruix, M.& Ribó, M. "Towards Tricking a Pathogen’s Protease into Fighting Infection: The 3D Structure of a Stable Circularly Permuted Onconase Variant Cleavedby HIV-1 Protease." PLoS One 8 (2013): e54568-e54568. Vert, A.; Castro, J.; Ruiz-Martínez, S.; Tubert, P., Escribano, D.; Ribó, M.; Vilanova, M.; and Benito, A. "Generation of New Cytotoxic Human Ribonuclease Variants Directed to the Nucleus." Molecular Pharmaceutics 9 (2012): 2894-2902. Castro, Jessica; Ribó, Marc; Puig, Teresa, Colomer, Ramon, Vilanova, Maria & Benito, Antoni "A cytotoxic ribonuclease reduces the expression level of P-glycoprotein in multidrug-resistant cell lines." Investigational New Drugs 30 (2012): 880-888. Tubert, Pere; Rodríguez, Montserrat; Ribó, Marc; Benito, Antoni; Vilanova, Maria "The nuclear transport capacity of a human-pancreatic ribonuclease variant is critical for its cytotoxicity." Investigational New Drugs 29 (2011): 811-817. Ercole, Carmine; López Alonso, Jorge Pedro; Font, Josep; Ribó, Marc; Vilanova, Maria; Picone, Delia; Laurents, Douglas V. "Crowding agents and osmolytes provide insight into the formation and dissociation of RNase A oligomers." Archives of Biochemistry and Biophysics 506 (2011): 123-129. Tubert, P., Laurents, D.V., Ribó, M., Bruix, M., Vilanova, M. & Benito, A. "Interactions crucial for 3D swapping in the HP-RNase variant PM8." Biophysical Journal 101 (2011): 459-467. Jessica Castro, Marc Ribó, Susanna Navarro, Maria Victòria Nogués, Maria Vilanova and Antoni Benito "A human ribonuclease induces apoptosis associated with p21WAF1/CIP1 induction and JNK inactivation." BMC Cancer 11 (2011): 9-. López Alonso, Jorge; Bruix, Marta; Font, Josep; Ribó, Marc; Vilanova, Maria; Jimenez, M. Angeles; Santoro, Jorge; González, Carlos; Laurents, Douglas "NMR Spectroscopy Reveals that RNase A is Chiefly Denatured in 40% Acetic Acid: Implications for Oligomer Formation by 3D Domain Swapping." Journal of the American Chemical Society 132 (2010): 1621-1630. Vilà, R.; Benito, A.; Ribó, M.; Vilanova, M. "Mapping the stability clusters in bovine pancreatic ribonuclease A ." Biopolymers (títol associat amb Peptide Science) 91 (2009): 1038-1047. Kurpiewska, K.; Font, J.; Ribó, M.; Vilanova, M.; Lewinski, K. "X-ray crystallographic studies of RNase A variants engineered at the most destabilizing positions of the main hydrophobic core: Further insight into protein stability." Proteins 77 (2009): 658-669. Marchal, S.; Font, J.; Ribó, M.; Vilanova, M.; Phillips, R.; Lange, R.; Torrent, J. "Asymmetric kinetics of protein structural changes." Accounts of Chemical Research 42 (2009): 778-787. Torrent, G.; Ribó, M.; Benito, A.; Vilanova, M. "Bactericidal activity engineered on human pancreatic ribonuclease and onconase." Molecular Pharmaceutics 6 (2009): 531-542. López-Alonso, J.P.; Diez-García, F.; Font, J.; Ribó, M.; Vilanova, M.; Scholtz, J.M.; González, C.; Vottariello, F.; Gotte, G.; Libonati, M.; Laurents, D.V. "Carbodiimide EDC induces cross-links that stabilize RNAse A C-dimer against dissociation: EDC adducts can affect protein net charge, conformation and activity." Bioconjugate Chemistry 20 (2009): 1459-1473. Vila-Perelló, M.; Hori, Y.; Ribó, M.; Muir, T.W. "Activation of protein splicing by protease or light triggered O to N acyl migration." Angewandte Chemie International Edition 47 (2008): 7764-7767. Torrent, G.; Benito, A.; Castro, J.; Ribó, M.; Vilanova, M. "Contribution of the C30/C75 disulfide bond to the biological properties of onconase." Biological Chemistry Hoppe-Seyler 389 (2008): 1127-1136. Benito, A.; Laurents, D.V.; Ribó, M.; Vilanova, M. "The structural determinants that lead to the formation of particular oligomeric structures in the pancreatic-type ribonuclease family." Current Protein and Peptide Science 9 (2008): 370-393. Bruix, M.; Ribó, M.; Benito, A.; Laurents, D.V.; Rico, M.; Vilanova, M. "Destabilizing mutations alter the hydrogen exchange mechanism of RNase A." Biophysical Journal 94 (2008): 2297-2305. Torrent, J.; Marchal, S.; Ribó, M.; Vilanova, M.; Georges, C.; Dupont, Y.; Lange, R. "Distinct unfolding and refolding pathways of ribonuclease A revealed by heating and cooling temperature jumps ." Biophysical Journal 94 (2008): 4056-4065. Benito, A.; Vilanova, M.; Ribó, M. "Intracellular routing of cytotoxic pancreatic-type ribonucleases ." Current Pharmaceutical Biotechnology 9 (2008): 169-179. Rodríguez, M.; Torrent, G.; Bosch, M.; Rayne, F.; Dubremetz, J.F.; Ribó, M.; Benito, A.; Vilanova, M.; Beaumelle, B. "Intracellular pathway of onconase that enables its delivery to the cytosol." Journal of Cell Science 120 (2007): 1405-1411. Font, J.; Torrent, J.; Ribó, M.; Laurents, D.V.; Balny, C.; Vilanova, M.; Lange, R. "Pressure-jump-induced kinetics reveals a hydration dependent folding/unfolding mechanism of ribonuclease A." Biophysical Journal 91 (2006): 2264-2274. Rodríguez, M.; Benito, A.; Tubert, P.; Castro, J.; Ribó, M.; Beaumelle, B.; Vilanova, M. "A cytotoxic ribonuclease variant with a discontinuous nuclear localization signal constituted by basic residues scattered over three areas of the molecule." Journal of Molecular Biology 360 (2006): 548-557. Font, J.; Benito, A.; Lange, R.; Ribó, M.; Vilanova, M. "The contribution of the residues from the main hydrophobic core of ribonuclease A to its pressure-folding transition state.." Protein Science 15 (2006): 1000-1009. Torrent, J.; Font, J.; Herberhold, H.; Marchal, S.; Ribó, M.; Ruan, K.; Winter, R.; Vilanova, M; Lange, R. "The use of pressure-jump relaxation kinetics to study protein folding landscapes." Biochimica et Biophysica Acta 1764 (2006): 489-496. López-Alonso, J.P.; Bruix, M.; Font, J.; Ribó, M.; Vilanova, M.; Rico, M.; Gotte, G.; Libonati, M.; González, C.; Laurents, D.V. "Formation, structure, and dissociation of the ribonuclease S three-dimensional domain-swapped dimer." Journal of Biological Chemistry 281 (2006): 9400-9406. Ribó, M.; Font, J.; Benito, A.; Torrent. J.; Lange, R.; Vilanova, M. "Pressure as a tool to study protein-unfolding/refolding processes: the case of ribonuclease A." Biochimica et Biophysica Acta 1764 (2006): 461-469. Rodríguez, M.; Benito, A.; Ribó, M.; Vilanova, M. "Characterization of the dimerization process of a domain-swapped dimeric variant of human pancreatic ribonuclease." Febs Journal 273 (2006): 1166-1176. Nikolovski, Z.; Buzón, V.; Ribó, M.; Moussaoui, M.; Vilanova, M.; Cuchillo, C.M.; Cladera, J.; Nogués, M.V. "Thermal unfolding of eosinophil cationic protein/ribonuclease 3: a nonreversible process." Protein Science 15 (2006): 2816-2827. Font, J.; Benito, A.; Torrent, J.; Lange, R.; Ribó, M.; Vilanova, M. "Pressure- and temperature-induced unfolding studies:Thermodynamics of core hydrophobicity and packing of ribonuclease A." Biological Chemistry Hoppe-Seyler 387 (2006): 285-296. Benito, A.; Ribó, M.; Vilanova, M. "On the track of antitumor ribonucleases." Molecular Biosystems 1 (2005): 294-302. Ribó, M.; Bosch, M.; Torrent, G.; Benito, A.; Beaumelle, B.; Vilanova, M. "Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation process of onconase." European Journal of Biochemistry 271 (2004): 1163-1171. Bosch, M.; Benito, A.; Ribó, M.; Puig, T.; Beaumelle, B.; Vilanova, M. "A nuclear localization sequence endows human pancreatic ribonuclease with cytotoxic activity." Biochemistry 43 (2004): 2167-2177. Benito, A.; Bosch, M.; Torrent, G.; Ribó, M.; Vilanova, M. "Stabilisation of human pancreatic ribonuclease through mutation at its N-terminal edge." Protein Engineering 15 (2002): 887-893. Canals, A.; Pous, J.; Guasch, A.; Benito, A.; Ribó, M.; Vilanova, M.; Coll, M. "The structure of an engineered domain-swapped ribonuclease dimer and its implications for the evolution of proteins towards oligomerization." Structure 9 (2001): 967-976. Ribó, M.; Benito, A.; Canals, A.; Nogués, M.V.; Cuchillo, C.M.; Vilanova, M. "Purification of engineered human pancreatic ribonuclease." Methods in Enzymology 341 (2001): 221-233. Torrent, J.; Rubens, P.; Ribó, M.; Heremans, K.; Vilanova, M. "Pressure versus temperature-unfolding of ribonuclease A: an FTIR spectroscopic characterization of 10 variants at the carboxy terminal site." Protein Science 10 (2001): 725-734. Pous, J.; Canals, A.; Terzyan, S.S.; Guasch, A.; Benito, A.; Ribó, M.; Vilanova, M.; Coll, M. "Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the dsign of cytotoxic ribonucleases." Journal of Molecular Biology 303 (2000): 49-59. Torrent, J.; Connelly, J.P.; Rubens, P.; Coll, M.G,; Ribó, M.; Lange, R.; Heremans, K.; Vilanova, M. "The response of the C-terminal variants of ribonuclease A to pressure and temperature-induced unfolding. A study by fourth derivative UV and Fourier transform infrared spectroscopy." High Pressure Research 19 (2000): 247-252. Canals, A., Ribó, M., Benito, A., Bosch, M., Mombelli, E. & Vilanova, M. "Production of engineered human pancreatic ribonucleases, solving expression and purification problems, and enhancing thermostability..." Protein Expression and Purification 17 (1999): 169-181. Coll, M.G., Protasevich, I.I., Torrent, J., Ribó, M., Lobachov, V.M., Makarov, A.A. & Vilanova, M. "Valine 108, a chain folding initiation site belonging residue, crucial for the ribonuclease A stability.." Biochemical and Biophysical Research Communications 265 (1999): 356-360. Torrent, J., Connelly, J.P., Coll, M.G., Ribó, M., Lange, R. & Vilanova, M. "Pressure versus heat-induced unfolding of ribonuclease A: The case of hydrophobic interactions within a chain folding initiation site.." Biochemistry 38 (1999): 15952-15961. Boqué, Ll., Coll, M.G., Ribó, M., Cuchillo, C.M., Fita, I. & Vilanova, M. "Tridimensional structure of three ribonuclease A covalent derivatives with pyridoxal 5'-phosphate.." Protein and Peptide Letters 5 (1998): 101-108. Nogués, M.V., Moussaoui, M., Boix, E., Vilanova, M. Ribó, M. & Cuchillo, C.M. "The contribution of noncatalytic phosphate binding subsites to the mechanism of bovine pancreatic ribonuclease A.." Cellular and Molecular Life Sciences 54 (1998): 766-774. Matousek, J., Kim, J-S, Soucek, J., Riha, J., Ribó, M., Leland, P.A. and Raines, R.T. "Ribonucleases endowed with specific toxicity for spermatogenic layers." Comparative Biochemistry and Physiology. Part B, Biochemistry and molecular biology 118B (1997): 881-888. Ribó, M., delCardayré, S.B., Raines, R. T, Llorens, R and Cuchillo, Claudi M. "Production of human pancreatic ribonuclease in Saccharomyces cerevisiae and Escherichia coli." Protein Expression and Purification 7 (1996): 253-256. delCardayré, S.B., Ribó, M., Yokel, E.M., Quirk, D.J., Rutter, W. J. and Raines, R. T. "Ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln 11." Protein Engineering 8 (1995): 261-273. Ribó, M., Beintema, J. J., Osset, M., Fernández, E., Bravo, J., Llorens, R. and Cuchillo C. M. "Heterogeneity in the Glycosylation Pattern of Human Pancreatic Ribonuclease. ." Biological Chemistry Hoppe-Seyler 375 (1994): 357-363. Bravo, J., Fernández, E., Ribó, M., Llorens, R. and Cuchillo C. M. "A versatile negative staining ribonuclease zymogram." Analytical Biochemistry 219 (1994): 82-86.
M. Ribó, E. Fernández, J. Bravo, M. Osset, M. J. M. Fallon, R. De Llorens and Claudi M. Cuchillo "Purification of human pancratic ribonuclease by high performance liquid chromatography." Structure, Mechanism and Function of Ribonucleases (1991): 157-162. M. J. M. Fallon, C. Balagué, E. Fernández, M. Ribó, J. Bravo, R. de Llorens and Claudi M. Cuchillo "Monoclonal Antibodies to Bovine Pancreatic Ribonuclease." Structure, Mechanism and Function of Ribonucleases (1991): 229-234. E. Fernández, M. Ribó, J. Bravo, M. Osset, M. J. M. Fallon, R. De Llorens and Claudi M. Cuchillo "Isolation of secreted enzymes with ribonuclease activity from human pancreatic adenocarcinoma cell line CAPAN-1." Structure, Mechanism and Function of Ribonucleases (1991): 163-168. J. Bravo, M. J. M. Fallon, M. Ribó, E. Fernández, M. Osset, R. De Llorens and Claudi M. Cuchillo "PHMB (p-hydroxymercuribenzoate) utilization to inactivate the inhibitor of ribonuclease in normal human pancreatic tissue and tissue culture medium from CAPAN-1 cells." Structure, Mechanism and Function of Ribonucleases (1991): 201-206.
Castro J; Ribo M; Benito A; Vilanova M "Apoptin, a Versatile Protein with Selective Antitumor Activity." Current Medicinal Chemistry (2018): -.