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Research Technical Services

Confocal Microscopy

Technique of microscopic observation

It Confocal Laser Microscopy it is a technique of observation microscopic that he founds on obtaining images of the emission from fluorescent light on the part of the sample, of which the light is eliminated from the plans out of focus, retiring only the light of the plan focal. This tea great advantages about the conventional microscopy, as more contrast, clarity and resolution of the images, and the possibility to achieve optic sections of the sample to carry out threedimensional studies.

Job request

In order to request that a sample be analysed, you need to contact the staff responsible for the team.

You will need to give the toxicity and any storage precautions. Once the report has been delivered, if you do not collect the sample within one week, any remaining sample will be destroyed.

The equipment

NIKON A1R

Year of acquisition:
2013
Module of spectral confocal laser microscopy (CLSM) installed in an inverted microscope motorised, of high benefits, endowed with:

- Bedframe of lasers

- Module of scanning

- Motorised platen

- Modules of control of the photomultiplier detectors and     spectral

- System of cell incubation

- Programme of control NIKON NIS-Elements AR v 4.10


Approximate resolution:
150 nms in XY and 250 nms in Z

It is a matter of a module of spectral confocal laser microscopy (CLSM) and of a module of microscopy of light structured of type "Spinning disk". Both modules have settled on an inverted microscope motorised of high benefits.

How does it work?

Technical characteristics of the instrumental whole:

  1. Inverted microscope NIKON Ti Eclipse motorised, with 6 objective Apocromàtics Plan (10x, 20x, 40x, 60x water, 60x oil, 100x oil), contrast of phases and I SUGGEST. Piezoelectric platen even of 1nm of resolution in the axis Z.
  2. Systems of lighting: source of light transmitted halògena minim, epifluorescència for optical fibre and turret for six blocks of filters, laser of argon with 4 lines of emission (457, 477, 488 and 514nms), laser of Helium-Neon (543nms), laser of diode UV (405nms) and laser of red diode (642nms).
  3. Confocal module NIKON A1R with galvanomètric tracking and resonant, of different directionalities. Maximum resolution of 4096 x 4096 pixels and speed limit of 420 fps in 512 x 32 pixels.
  4. Detectors of reflected light: 4 photomultipliers and spectral module of 32 canals.
  5. Module of spinning disk DSD ANDOR of structured lighting and camera CCD of high definition.
  6. System of cell incubation of overplaten, with atmosphere of CO2, endowed with control of temperature and of humidity.
  7. Ordinador  HP Z 820 for the complete control of the whole instrumental and of capture of images Software owner NIS Elements v 4.10
  8. Independent computer to be able to work "off line", destined to the analysis of images on the part of the users.

Applications / Practical cases

Possibility to carry out different essays of fluorescence in alive cell: FRAP, experiments of type, BREAD, FRET Time lapse, studies of colocation..., and also by means of reflection, observing the structure of materials, pores and microfisures, roughness, three-dimensional fibre studes, and so on.

web images

More information:

All those that you are interested on receiving information more specific to the team and its applications, you can put in touch with the responsibles for the team.

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