1. General introduction to the recombinant DNA technology. Applications. Hosts to produce recombinant proteins. Transgenic organisms (pharming) and control of transgene expression. CRISPR/Cas system and its applications.
2. How to get improved recombinant proteins of therapeutic interest. Mutagenesis. Classification. Directed evolution and screening methods: Phage display, ribosome display...etc.
3. Application of the methodologies described. Some examples of therapeutic interest: L-Asparaginase and variants of monoclonal antibody Herceptin (against HER2). Modular drugs. Are the “magic bullets" possible? General discussion and examples.
4. Recombinant protein production. Strategies for the purification of recombinant proteins. Levels of production: scaling.
5. Biotechnological products of therapeutic interest. Potential systems to produce recombinant proteins. Production in eukaryotic cells: design of vectors and host cell properties.
6. Development of stable cell lines for the production of therapeutic recombinant proteins. Assays and selection of highly producing cell clones. Establishment of a cell bank, master cell bank and working cell bank. Upstream processing: Types and strategies of fermentation processes. Downstream processing: separation and purification.
Written reports and oral presentations will be individual whenever possible. If it is necessary to work in teams the final mark of the written report will be the same for all the team members unless negligence is detected.
In the oral presentation it is obligatory to specify which part of the work has done each team member independently of the organization of the exposition.
Criteris específics de la nota «No Presentat»:
The qualification will be NOT PRESENTED if the written report is not presented and/or the oral presentation is not given. It is mandatory the assitance to a minimum of the 80% of the classes including the presentation of the rest of classmates.